Rapid, Easy and Reliable Sexing Method for Falconiformes by PCR

Rapid, easy and reliable calibration for freehand 3D ultrasound.

This paper presents improvements to the plane-based technique for calibrating freehand 3D ultrasound systems. The improvements are designed to make it easier for inexperienced users to perform plane-based calibration and to know that they have got a reliable result. In particular, we enable the calibration to be performed using water at room temperature while producing a result that is valid fo...

Rapid and reliable cloning of PCR products.

CRISPR-STAT: an easy and reliable PCR-based method to evaluate target-specific sgRNA activity

CRISPR/Cas9 has emerged as a versatile genome-engineering tool that relies on a single guide RNA (sgRNA) and the Cas9 enzyme for genome editing. Simple, fast and economical methods to generate sgRNAs have made targeted mutagenesis routine in cultured cells, mice, zebrafish and other model systems. Pre-screening of sgRNAs for target efficacy is desirable both for successful mutagenesis and minim...

A rapid and reliable PCR method for detecting clonal T cell populations.

Aims-To establish a reverse transcription polymerase chain reaction (RT-PCR) for the detection of clonal T cell populations, and to evaluate the sensitivity and reliability of the technique.Methods-After reverse transcription of the target RNA with a consensus T cell receptor (TCR) beta constant (C) region primer, consensus C, variable (V), diversity (D) and joining (J) region primers were used...

Comparing Rapid and Specific Detection of Brucella in Clinical Samples by PCR-ELISA and Multiplex-PCR Method

Background: Rapid diagnosis and differentiation of Brucella is of high importance due to the side effects of antibiotics for the treatment of brucellosis. This study aimed to identify and compare PCR-ELISA as a more accurate diagnositc test with other common molecular and serological tests. Methods: ...